IJLST 2018 Volume 11 Issue 2

International Journal of Life Sciences and Technology (IJLST) ISSN: 0974-5335

IJBST Journal Group -- Open Access -- NO Fees -- NO Processing Charges -- 100% Non Profit Initiatives

The IJBST Journal Group subscribes to the San Francisco Declaration on Research Assessment and the The European Code of Conduct for Research Integrity

The IJBST Journal Group Archive can also be accessed at https://archive.org/details/IJBSTJournalGroup

A Forensic view: Quality Control Norms for Analytical Instruments. Nilimamayee Samal, A.C. Rajvanshi. IJLST (2018), 11(2):15-19

Full Text PDF:


A Forensic view: Quality Control Norms for Analytical Instruments


Nilimamayee Samal, A.C. Rajvanshi

Lok Nayak Jayaprakash Narayan National Institute of Criminology and Forensic Science, New Delhi, INDIA

nilima.samal1@gmail.com, aviraj29@gmail.com


A wide variety of Analytical Instruments are available for identification and quantification of various drugs and poisons in Forensic Toxicology Laboratories. The LC-MS/MS (Liquid Chromatography- Mass Spectrometer with tandem Mass) is an emerging technique in the field of Forensic Toxicology, which provides the ability to analyze a high range molecular weight; high polarity range of compounds with high sensitivity and specificity, in less time. But in case of LC-MS or LC-MS/MS, many factors influence the mass spectra of organic molecule during analysis. The collision induced dissociation of organic molecules by Atmospheric Pressure Chemical Ionization (APCI) or Electro-Spray Ionization (ESI) show the need of standardization of experimental conditions for building up a reference library. The different mass filters like Time of Flight (TOF), Ion Trap, Single or Triple Quadruple, Magnetic Sector Mass Analyzers in single or multiple mass spectrometry coupled with High Performance Liquid Chromatography(HPLC) give their unique pattern of mass spectra which are not easy to compare with each other. So, when confirmation of identity of a compound is required, it is very difficult to rely on the definite set of library data. In this article, the attention on the above problem is directed explicitly to Forensic Toxicology. The standard protocol that should be followed and the number of Identification Points or Information Points (IPs) that should be taken into account for confirmation of identity of a chemical compound by LC-MS/MS has been discussed.

Keywords: Analytical Instrumentation, LC-MS/MS, Quality Criteria, Identification Point, Forensic Toxicology.