IJLST 2016 Volume 9 Issue 4
International Journal of Life Sciences and Technology (IJLST) ISSN: 0974-5335
An Open Access Journal -- NO Fees -- NO Processing Charges -- 100% Non Profit Initiatives
Anticlastogenic and antioxidant activity of Cassia fistula against Cyclophosphamide induced micronucleus formation in Swiss albino mice. Maya Kushwaha and Ramesh C Agrawal. IJLST (2016), 9(4):36-43
Title:
Anticlastogenic and antioxidant activity of Cassia fistula against Cyclophosphamide induced micronucleus formation in
Swiss albino mice
Authors & Affiliation:
Maya Kushwaha and Ramesh C Agrawal
Dept of Bioscience, Barkatullah University, Bhopal
Department of Research, Priyamvada Birla Cancer Research Institute, Birla Hospital
kushwahamaya21@gmail.com, rcagrawal60@yahoo.com
Abstract:
Traditional Systems of medicines play an important role in global health care needs. More than 30% of the entire plant species are used for medicinal purposes. Purpose: The present investigation was undertaken to investigate the Anticlastogenic effect and antioxidant property of Cassia fistula (CF) extract against Cyclophosphamide (CP) induced micronucleus formation in the mouse bone marrow cells. Methods: The bone marrow micronucleus assay was undertaken for anticlastogenic activity However the fenton reaction method was used to study the antioxidant activity . Results: The single IP administration of CF seed extract at the dose of 500, 1000 and 1500 mg/kg body weight, 24 hours prior the administration of CP (at the dose of 50 mg/kg) have significantly prevented the micronucleus formations in dose dependent manner in bone marrow cells of mice as compared to CP group. In another experiment an in vitro antioxidant assay which is based on the oxidative damage to 2-deoxy-D-ribose induced by hydroxyl radicals (•OH) generated by the reaction between ascorbic acid and (Fe III)-EDTA has been examined. Conclusion : Our results suggest that Cassia fistula have a preventive potential against CP-induced micronucleus formation in Swiss mouse bone marrow cells probably due to its antioxidant properties.
Keywords: Cassia fistula, Mutagenicity, Micronucleus, Bone marrow, Cyclophosphamide.