IJLST 2016 Volume 9 Issue 11

International Journal of Life Sciences and Technology (IJLST) ISSN: 0974-5335

An Open Access Journal -- NO Fees -- NO Processing Charges -- 100% Non Profit Initiatives

Effects of D-003 and Lyprinol on acetic acid writhing test in mice. Vivian Molina, Zullyt Zamora, Licet Mena. IJLST (2016), 9(11):88-95


Effects of D-003 and Lyprinol on acetic acid writhing test in mice

Authors & Affiliation:

Vivian Molina 1, Zullyt Zamora2, Licet Mena3.

1-Titular Researcher, Dr. Pharmaceutical Sciences, Head of the Pharmacology and Toxicology Department,

2-Auxiliar Research, Dr. Health Sciences, Pharmacology and Toxicology Department,

3- Aspiring Researcher, Pharmacology and Toxicology Department,

Centre of Natural Products, National Centre for Scientific Research, Havana, Cuba

vivian.molina@cnic.edu.cu, zullyt.zamora@cnic.edu.cu, licet.mena@cnic.edu.cu


Pain is annoying symptom of various acute and chronic inflammatory disorders affecting the quality of life of suffers. Its treatments include anti-inflammatory drugs with adverse effects as gastrointestinal or cardiovascular disorders. Therefore, the search for more secure and effective analgesic drugs is updated. This study investigated the effects of D-003, Lyprinol and the combination (D-003 + Lyprinol) on acetic acid writhing test in mice. Two experimental series were performed. The first evaluated different doses of D-003 (5, 25, 100, 200, 400 and 800 mg/kg) and Lyprinol (5, 25, 100, 200 and 400 mg/kg) on acetic acid-induced writhing in mice, while the second one evaluated the combination therapy D-003 (25 mg/kg) + Lyprinol (25 mg/kg) in this test. Ibuprofen (100 mg/kg) as a reference drug was evaluated too. D-003 (5, 25, 100, 200, 400 and 800 mg/kg) significantly, markedly and dose dependently inhibited the number of writhings in mice compared to the control group (29.3, 33.8, 42, 58.7, 72.9 and 73.7 %, respectively). Lyprinol (25, 100, 200 and 400 mg/kg) produced a significant, modest and non-dose-dependent inhibition of the writhings number compared with the control group (36.3, 35.2, 36.1 and 35.3%, respectively. Ibuprofen (100 mg/kg) marked and significantly inhibited the writhes (56.1%). Comparison of equal doses of D-003 and Lyprinol showed statistically significant differences at the doses of 200 and 400 mg kg, showing greater percent inhibition with D-003 (73%) than with Lyprinol (36%). The combination of D-003+Lyprinol caused a marked inhibition (67.3%) that was statistically significant not only compared with the control group, but with respect to separate D-003 (34.8 %) and Lyprinol (27.9 %), respectively. In conclusion, D-003, Lyprinol and the combined therapy D-003+Lyprinol significantly inhibited acetic acid-induced writhing number in mice, while the combined therapy produced an additional benefit compared to monotherapies with an additive-type positive interaction.

Key words: D-003, Lyprinol, combined therapy, pain, acetic acid-writhing test, mice.