IJLST 2016 Volume 9 Issue 11
International Journal of Life Sciences and Technology (IJLST) ISSN: 0974-5335
An Open Access Journal -- NO Fees -- NO Processing Charges -- 100% Non Profit Initiatives
Effects of D-003 and Lyprinol on acetic acid writhing test in mice. Vivian Molina, Zullyt Zamora, Licet Mena. IJLST (2016), 9(11):88-95
Effects of D-003 and Lyprinol on acetic acid writhing test in mice
Authors & Affiliation:
Vivian Molina 1, Zullyt Zamora2, Licet Mena3.
1-Titular Researcher, Dr. Pharmaceutical Sciences, Head of the Pharmacology and Toxicology Department,
2-Auxiliar Research, Dr. Health Sciences, Pharmacology and Toxicology Department,
3- Aspiring Researcher, Pharmacology and Toxicology Department,
Centre of Natural Products, National Centre for Scientific Research, Havana, Cuba
Pain is annoying symptom of various acute and chronic inflammatory disorders affecting the quality of life of suffers. Its treatments include anti-inflammatory drugs with adverse effects as gastrointestinal or cardiovascular disorders. Therefore, the search for more secure and effective analgesic drugs is updated. This study investigated the effects of D-003, Lyprinol and the combination (D-003 + Lyprinol) on acetic acid writhing test in mice. Two experimental series were performed. The first evaluated different doses of D-003 (5, 25, 100, 200, 400 and 800 mg/kg) and Lyprinol (5, 25, 100, 200 and 400 mg/kg) on acetic acid-induced writhing in mice, while the second one evaluated the combination therapy D-003 (25 mg/kg) + Lyprinol (25 mg/kg) in this test. Ibuprofen (100 mg/kg) as a reference drug was evaluated too. D-003 (5, 25, 100, 200, 400 and 800 mg/kg) significantly, markedly and dose dependently inhibited the number of writhings in mice compared to the control group (29.3, 33.8, 42, 58.7, 72.9 and 73.7 %, respectively). Lyprinol (25, 100, 200 and 400 mg/kg) produced a significant, modest and non-dose-dependent inhibition of the writhings number compared with the control group (36.3, 35.2, 36.1 and 35.3%, respectively. Ibuprofen (100 mg/kg) marked and significantly inhibited the writhes (56.1%). Comparison of equal doses of D-003 and Lyprinol showed statistically significant differences at the doses of 200 and 400 mg kg, showing greater percent inhibition with D-003 (73%) than with Lyprinol (36%). The combination of D-003+Lyprinol caused a marked inhibition (67.3%) that was statistically significant not only compared with the control group, but with respect to separate D-003 (34.8 %) and Lyprinol (27.9 %), respectively. In conclusion, D-003, Lyprinol and the combined therapy D-003+Lyprinol significantly inhibited acetic acid-induced writhing number in mice, while the combined therapy produced an additional benefit compared to monotherapies with an additive-type positive interaction.
Key words: D-003, Lyprinol, combined therapy, pain, acetic acid-writhing test, mice.